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Insulin secretions in cells INS-1 832/3 SNAP/FLAG-hGLP-1R WT or V229A cells were seeded onto 48-well plates, pre- incubated in 3 mM glucose media overnight, washed with KRBH buffer and incubated in 3 mM glucose KRBH buffer for 1 hour before stimulation with 11 mM glucose KRBH +/- 100 nM exendin-4 for 1 hour

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Here is a week-by-week protocol that combines the strategies from this guide into a practical action plan